BioWrite™ micropatterned substrates

Below are images of BioWrite™ protein micropatterned substrates made by Intelligent Substrates. Click on the images for full size versions.

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HUVECs on 50 µm fibronectin squares (green). Actin is red. Nuclei are blue.
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HUVECs on 50 µm grid of fibronectin (green). Actin is red. Nuclei are blue.
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Single HUVEC on 50 µm fibronectin square (green). Actin is red. Nuclei are blue.
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CHO cells on 35 µm lines of fibronectin. Actin is red, nuclei are blue.
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Swiss-3T3 cells confined to 50 µm FN square patterns. Actin is red.
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Swiss-3T3 cells align to 30 µm FN line patterns. Actin is red.
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WI-38 fibroblasts on 70 µm lines of fibronectin. Actin is red, nuclei are blue.
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Swiss-3T3 cells confined to 50 µm fibronectin squares. Cell membrane is red, nuclei are blue.
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Swiss-3T3 cells confined to a grid of 50 µm squares. Cell membrane is red. Unconfined cells vary in size, shape, and location.
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MDA-MB-231 breast cancer cells on 10 µm lines of fibronectin.
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Swiss-3T3 fibroblasts on 20 µm lines of collagen.
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Phase image of Swiss-3T3 cells on 50 µm fibronectin squares.
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Swiss-3T3 cells on an array of 5 µm fibronectin squares. Actin is blue, and vinculin (focal adhesions) is green.
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Swiss 3T3 fibroblasts on fibronectin-patterned PDMS.
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Swiss 3T3 fibroblasts on 50 µm collagen squares.
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Swiss 3T3 fibroblasts on fibronectin diamonds. Actin is green, and nucleus is blue.
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Swiss 3T3 fibroblasts on fibronectin triangles. Actin is green, and nucleus is blue.
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Complex fibronectin pattern.
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MCF7 breast cancer cell stradling 40 µm squares of fibronectin (green). Actin is red, and nucleus is blue.
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Swiss-3T3 fibroblasts on 10 µm squares of fibronectin.
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HUVECs on 50 µm wide lines of fibronectin.
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Swiss 3T3 fibroblasts on fibronectin grids, line widths are 8 microns.