Bibliography

Protein micropatterned substrates are emerging as powerful tools for biomedical research. We assembled a collection of articles spanning four decades describing how scientists have used micropatterned substrates to gain new insights into cell biology. We focused mainly on papers reporting biological results versus papers describing new methods to produce micropatterns for cell culture. This is not a complete list -- please contact us if you wish to suggest papers to include in this resource.

2013
Pan CJ, Ding HY, Dong YX.
Extracellular matrix protein patterns guide human chondrocytes adhesion and alignment characterized by vimentin and matrilin-3.
Colloids Surf B Biointerfaces 102:730–736. 2013. More

Shulman M, Nahmias Y.
Long-term culture and coculture of primary rat and human hepatocytes.
Methods Mol Biol 945:287–302. 2013. More

Uhrin P, Breuss JM.
uPAR: A modulator of VEGF-induced angiogenesis.
Cell Adh Migr 7(1):23–26. 2013. More

March S, Ng S, Velmurugan S, Galstian A, Shan J, Logan DJ, Carpenter AE, Thomas D, Sim BK, Mota MM et al.
A Microscale Human Liver Platform that Supports the Hepatic Stages of Plasmodium falciparum and vivax.
Cell Host Microbe 14(1):104–115. 2013. More

Takahashi H, Shimizu T, Nakayama M, Yamato M, Okano T.
The use of anisotropic cell sheets to control orientation during the self-organization of 3D muscle tissue.
Biomaterials 34(30):7372–7380. 2013. More

Ukairo O, McVay M, Krzyzewski S, Aoyama S, Rose K, Andersen ME, Khetani SR, Lecluyse EL.
Bioactivation and Toxicity of Acetaminophen in a Rat Hepatocyte Micropatterned Coculture System.
J Biochem Mol Toxicol 2013. More

Varner VD, Nelson CM.
Let's push things forward: disruptive technologies and the mechanics of tissue assembly.
Integr Biol (Camb) 2013. More

Xu X, Wittenberg NJ, Jordan LR, Kumar S, Watzlawik JO, Warrington AE, Oh SH, Rodriguez M.
A patterned recombinant human IgM guides neurite outgrowth of CNS neurons.
Sci Rep 3:2267. 2013. More

Wang C, Baker BM, Chen CS, Schwartz MA.
Endothelial Cell Sensing of Flow Direction.
Arterioscler Thromb Vasc Biol 2013. More

Stiles JM, Pham R, Rowntree RK, Amaya C, Battiste J, Boucheron LE, Mitchell DC, Bryan BA.
Morphological restriction of human coronary artery endothelial cells substantially impacts global gene expression patterns.
FEBS J 2013. More

Wang Y, Xu Z, Kam LC, Shi P.
Site-Specific Differentiation of Neural Stem Cell Regulated by Micropatterned Multicomponent Interfaces.
Adv Healthc Mater 2013. More

Jain N, Iyer KV, Kumar A, Shivashankar GV.
Cell geometric constraints induce modular gene-expression patterns via redistribution of HDAC3 regulated by actomyosin contractility.
Proc Natl Acad Sci U S A 110(28):11349–11354. 2013. More

Doxzen K, Vedula SR, Leong MC, Hirata H, Gov NS, Kabla AJ, Ladoux B, Lim CT.
Guidance of collective cell migration by substrate geometry.
Integr Biol (Camb) 5(8):1026–1035. 2013. More

Leight JL, Liu WF, Chaturvedi RR, Chen S, Yang MT, Raghavan S, Chen CS.
Manipulation of 3D Cluster Size and Geometry by Release from 2D Micropatterns.
Cell Mol Bioeng 5(3):299–306. 2012. More

Wan LQ, Ronaldson K, Guirguis M, Vunjak-Novakovic G.
Micropatterning of cells reveals chiral morphogenesis.
Stem Cell Res Ther 4(2):24. 2013. More

Vonaesch P, Cardini S, Sellin ME, Goud B, Hardt WD, Schauer K.
Quantitative insights into actin rearrangements and bacterial target site selection from Salmonella Typhimurium infection of micropatterned cells.
Cell Microbiol 2013. More

Sun Y, Duffy R, Lee A, Feinberg AW.
Optimizing the structure and contractility of engineered skeletal muscle thin films.
Acta Biomater 9(8):7885–7894. 2013. More

Zouani OF, Lei Y, Durrieu MC.
Pericytes, Stem-Cell-Like Cells, but not Mesenchymal Stem Cells are Recruited to Support Microvascular Tube Stabilization.
Small 2013. More

Wang X, Song W, Kawazoe N, Chen G.
The osteogenic differentiation of mesenchymal stem cells by controlled cell-cell interaction on micropatterned surfaces.
J Biomed Mater Res A 2013. More

Zhong W, Li Y, Li L, Zhang W, Wang S, Zheng X.
YAP-mediated regulation of the chondrogenic phenotype in response to matrix elasticity.
J Mol Histol 2013. More

Atmanli A, Domian IJ.
Generation of aligned functional myocardial tissue through microcontact printing.
J Vis Exp (73):e50288. 2013. More

Pitaval A, Christ A, Curtet A, Tseng Q, Thery M.
Probing ciliogenesis using micropatterned substrates.
Methods Enzymol 525:109–130. 2013. More

Rodriguez-Fraticelli AE, Martin-Belmonte F.
Mechanical control of epithelial lumen formation.
Small GTPases 4(2):136–140. 2013. More

Ouyang M, Lu S, Kim T, Chen CE, Seong J, Leckband DE, Wang F, Reynolds AB, Schwartz MA, Wang Y.
N-cadherin regulates spatially polarized signals through distinct p120ctn and beta-catenin-dependent signalling pathways.
Nat Commun 4:1589. 2013. More

Kim MC, Neal DM, Kamm RD, Asada HH.
Dynamic modeling of cell migration and spreading behaviors on fibronectin coated planar substrates and micropatterned geometries.
PLoS Comput Biol 9(2):e1002926. 2013. More

Chang SS, Guo WH, Kim Y, Wang YL.
Guidance of cell migration by substrate dimension.
Biophys J 104(2):313–321. 2013. More

Liu W, Zheng W, Yuan B, Jiang X.
A micropatterned coculture system for axon guidance reveals that Slit promotes axon fasciculation and regulates the expression of L1CAM.
Integr Biol (Camb) 5(3):617–623. 2013. More

Zychowicz M, Mehn D, Ruiz A, Frontczak-Baniewicz M, Rossi F, Buzanska L.
Patterning of human cord blood-derived stem cells on single cell posts and lines: Implications for neural commitment.
Acta Neurobiol Exp (Wars) 72(4):325–336. 2012. More

Huang NF, Lai ES, Ribeiro AJ, Pan S, Pruitt BL, Fuller GG, Cooke JP.
Spatial patterning of endothelium modulates cell morphology, adhesiveness and transcriptional signature.
Biomaterials 34(12):2928–2937. 2013. More



2012
Kushiro K, Asthagiri AR.
Modular design of micropattern geometry achieves combinatorial enhancements in cell motility.
Langmuir 28(9):4357–4362. 2012. More

Yoon SH, Kim YK, Han ED, Seo YH, Kim BH, Mofrad MR.
Passive control of cell locomotion using micropatterns: the effect of micropattern geometry on the migratory behavior of adherent cells.
Lab Chip 12(13):2391–2402. 2012. More

Isenberg BC, Backman DE, Kinahan ME, Jesudason R, Suki B, Stone PJ, Davis EC, Wong JY.
Micropatterned cell sheets with defined cell and extracellular matrix orientation exhibit anisotropic mechanical properties.
J Biomech 45(5):756–761. 2012. More

Chen TH, Hsu JJ, Zhao X, Guo C, Wong MN, Huang Y, Li Z, Garfinkel A, Ho CM, Tintut Y et al.
Left-right symmetry breaking in tissue morphogenesis via cytoskeletal mechanics.
Circ Res 110(4):551–559. 2012. More

Alexander RA, Prager GW, Mihaly-Bison J, Uhrin P, Sunzenauer S, Binder BR, Schutz GJ, Freissmuth M, Breuss JM.
VEGF-induced endothelial cell migration requires urokinase receptor (uPAR)-dependent integrin redistribution.
Cardiovasc Res 94(1):125–135. 2012. More

Versaevel M, Grevesse T, Gabriele S.
Spatial coordination between cell and nuclear shape within micropatterned endothelial cells.
Nat Commun 3:671. 2012. More

Yu X, Cohen DM, Chen CS.
miR-125b Is an adhesion-regulated microRNA that protects mesenchymal stem cells from anoikis.
Stem Cells 30(5):956–964. 2012. More

Liazoghli D, Roth AD, Thostrup P, Colman DR.
Substrate Micropatterning as a New in Vitro Cell Culture System to Study Myelination.
ACS Chem Neurosci 3(2):90–95. 2012. More

Guo WH, Wang YL.
A three-component mechanism for fibroblast migration with a contractile cell body that couples a myosin II-independent propulsive anterior to a myosin II-dependent resistive tail.
Mol Biol Cell 23(9):1657–1663. 2012. More

Garcia-Parra P, Cavaliere F, Maroto M, Bilbao L, Obieta I, Lopez de Munain A, Alava JI, Izeta A.
Modeling neural differentiation on micropatterned substrates coated with neural matrix components.
Front Cell Neurosci 6:10. 2012. More

Loosli Y, Vianay B, Luginbuehl R, Snedeker JG.
Numerically bridging lamellipodial and filopodial activity during cell spreading reveals a potentially novel trigger of focal adhesion maturation.
Integr Biol (Camb) 4(5):508–521. 2012. More

Grosberg A, Nesmith AP, Goss JA, Brigham MD, McCain ML, Parker KK.
Muscle on a chip: in vitro contractility assays for smooth and striated muscle.
J Pharmacol Toxicol Methods 65(3):126–135. 2012. More

Takayama Y, Kotake N, Haga T, Suzuki T, Mabuchi K.
Formation of one-way-structured cultured neuronal networks in microfluidic devices combining with micropatterning techniques.
J Biosci Bioeng 114(1):92–95. 2012. More

Howk CL, Levine HA, Smiley MW, Mallapragada SK, Nilsen-Hamilton M, Oh J, Sakaguchi DS.
A mathematical model for selective differentiation of neural progenitor cells on micropatterned polymer substrates.
Math Biosci 238(2):65–79. 2012. More

Moller J, Luehmann T, Hall H, Vogel V.
The race to the pole: how high-aspect ratio shape and heterogeneous environments limit phagocytosis of filamentous Escherichia coli bacteria by macrophages.
Nano Lett 12(6):2901–2905. 2012. More

Feinberg AW, Alford PW, Jin H, Ripplinger CM, Werdich AA, Sheehy SP, Grosberg A, Parker KK.
Controlling the contractile strength of engineered cardiac muscle by hierarchal tissue architecture.
Biomaterials 33(23):5732–5741. 2012. More

Tong Z, Cheung LS, Stebe KJ, Konstantopoulos K.
Selectin-mediated adhesion in shear flow using micropatterned substrates: multiple-bond interactions govern the critical length for cell binding.
Integr Biol (Camb) 4(8):847–856. 2012. More

Janardhanan S, Wang MO, Fisher JP.
Coculture strategies in bone tissue engineering: the impact of culture conditions on pluripotent stem cell populations.
Tissue Eng Part B Rev 18(4):312–321. 2012. More

Peng R, Yao X, Cao B, Tang J, Ding J.
The effect of culture conditions on the adipogenic and osteogenic inductions of mesenchymal stem cells on micropatterned surfaces.
Biomaterials 33(26):6008–6019. 2012. More

Lei Y, Zouani OF, Remy M, Ayela C, Durrieu MC.
Geometrical microfeature cues for directing tubulogenesis of endothelial cells.
PLoS One 7(7):e41163. 2012. More

Planaguma J, Minsaas L, Pons M, Myhren L, Garrido G, Aragay AM.
Filamin A-Hinge Region 1-EGFP: A Novel Tool for Tracking the Cellular Functions of Filamin A in Real-Time.
PLoS One 7(8):e40864. 2012. More



2011
Wan LQ, Vunjak-Novakovic G.
Micropatterning chiral morphogenesis.
Commun Integr Biol 4(6):745–748. 2011. More

Kita A, Sakurai Y, Myers DR, Rounsevell R, Huang JN, Seok TJ, Yu K, Wu MC, Fletcher DA, Lam WA.
Microenvironmental geometry guides platelet adhesion and spreading: a quantitative analysis at the single cell level.
PLoS One 6(10):e26437. 2011. More

Li X, Chu J, Wang A, Zhu Y, Chu WK, Yang L, Li S.
Uniaxial mechanical strain modulates the differentiation of neural crest stem cells into smooth muscle lineage on micropatterned surfaces.
PLoS One 6(10):e26029. 2011. More

Alford PW, Nesmith AP, Seywerd JN, Grosberg A, Parker KK.
Vascular smooth muscle contractility depends on cell shape.
Integr Biol (Camb) 2011. More

Halidi N, Boittin FX, Beny JL, Meister JJ.
Propagation of fast and slow intercellular Ca(2+) waves in primary cultured arterial smooth muscle cells.
Cell Calcium 2011. More

Hoffecker IT, Guo WH, Wang YL.
Assessing the spatial resolution of cellular rigidity sensing using a micropatterned hydrogel-photoresist composite.
Lab Chip 11(20):3538–3544. 2011. More

Liu Y, Yan J, Howland MC, Kwa T, Revzin A.
Micropatterned Aptasensors for Continuous Monitoring of Cytokine Release from Human Leukocytes.
Anal Chem 2011. More

Wang YK, Yu X, Cohen DM, Wozniak MA, Yang MT, Gao L, Eyckmans J, Chen CS.
Bone Morphogenetic Protein-2-Induced Signaling and Osteogenesis Is Regulated by Cell Shape, RhoA/ROCK, and Cytoskeletal Tension.
Stem Cells Dev 2011. More

Anderson DE, Hinds MT.
Endothelial cell micropatterning: methods, effects, and applications.
Ann Biomed Eng 39(9):2329–2345. 2011. More

Peng R, Yao X, Ding J.
Effect of cell anisotropy on differentiation of stem cells on micropatterned surfaces through the controlled single cell adhesion.
Biomaterials 32(32):8048–8057. 2011. More

Desai RA, Khan MK, Gopal SB, Chen CS.
Subcellular spatial segregation of integrin subtypes by patterned multicomponent surfaces.
Integr Biol (Camb) 3(5):560–567. 2011. More

Yan C, Sun J, Ding J.
Critical areas of cell adhesion on micropatterned surfaces.
Biomaterials 32(16):3931–3938. 2011. More

Kumar G, Co CC, Ho CC.
Steering cell migration using microarray amplification of natural directional persistence.
Langmuir 27(7):3803–3807. 2011. More

Tee SY, Fu J, Chen CS, Janmey PA.
Cell shape and substrate rigidity both regulate cell stiffness.
Biophys J 100(5):L25–7. 2011. More

Lee CH, Bose S, Van Vliet KJ, Karp JM, Karnik R.
Studying cell rolling trajectories on asymmetric receptor patterns.
J Vis Exp (48)2011. More

Stricker J, Sabass B, Schwarz US, Gardel ML.
Optimization of traction force microscopy for micron-sized focal adhesions.
J Phys Condens Matter 22(19):194104. 2010. More

Song W, Lu H, Kawazoe N, Chen G.
Adipogenic differentiation of individual mesenchymal stem cell on different geometric micropatterns.
Langmuir 27(10):6155–6162. 2011. More

Tsai IY, Kuo CC, Tomczyk N, Stachelek SJ, Composto RJ, Eckmann DM.
Human macrophage adhesion on polysaccharide patterned surfaces.
Soft Matter 7:3599–3606. 2011. More

Li Y, Chu JS, Kurpinski K, Li X, Bautista DM, Yang L, Sung KL, Li S.
Biophysical regulation of histone acetylation in mesenchymal stem cells.
Biophys J 100(8):1902–1909. 2011. More

Zychowicz M, Mehn D, Ruiz A, Colpo P, Rossi F, Frontczak-Baniewicz M, Domanska-Janik K, Buzanska L.
Proliferation capacity of cord blood derived neural stem cell line on different micro-scale biofunctional domains.
Acta Neurobiol Exp (Wars) 71(1):12–23. 2011. More

Tay CY, Pal M, Yu H, Leong WS, Tan NS, Ng KW, Venkatraman S, Boey F, Leong DT, Tan LP.
Bio-inspired micropatterned platform to steer stem cell differentiation.
Small 7(10):1416–1421. 2011. More

Van Hoof D, Mendelsohn AD, Seerke R, Desai TA, German MS.
Differentiation of human embryonic stem cells into pancreatic endoderm in patterned size-controlled clusters.
Stem Cell Res 6(3):276–285. 2011. More

Tseng Q, Wang I, Duchemin-Pelletier E, Azioune A, Carpi N, Gao J, Filhol O, Piel M, Thery M, Balland M.
A new micropatterning method of soft substrates reveals that different tumorigenic signals can promote or reduce cell contraction levels.
Lab Chip 11(13):2231–2240. 2011. More

Clarke JC, Tuft BW, Clinger JD, Levine R, Figueroa LS, Allan Guymon C, Hansen MR.
Micropatterned methacrylate polymers direct spiral ganglion neurite and Schwann cell growth.
Hear Res 2011. More

Corum LE, Eichinger CD, Hsiao TW, Hlady V.
Using microcontact printing of fibrinogen to control surface-induced platelet adhesion and activation.
Langmuir 27(13):8316–8322. 2011. More

Fereol S, Fodil R, Barnat M, Georget V, Milbreta U, Nothias F.
Micropatterned ECM substrates reveal complementary contribution of low and high affinity ligands to neurite outgrowth.
Cytoskeleton (Hoboken) 2011. More

Wan LQ, Ronaldson K, Park M, Taylor G, Zhang Y, Gimble JM, Vunjak-Novakovic G.
Micropatterned mammalian cells exhibit phenotype-specific left-right asymmetry.
Proc Natl Acad Sci U S A 2011. More



2010
Pitaval A, Tseng Q, Bornens M, Thery M.
Cell shape and contractility regulate ciliogenesis in cell cycle-arrested cells.
J Cell Biol 191(2):303–312. 2010. More

Blong CC, Jeon CJ, Yeo JY, Ye EA, Oh J, Callahan JM, Law WD, Mallapragada SK, Sakaguchi DS.
Differentiation and behavior of human neural progenitors on micropatterned substrates and in the developing retina.
J Neurosci Res 88(7):1445–1456. 2010. More

Kilian KA, Bugarija B, Lahn BT, Mrksich M.
Geometric cues for directing the differentiation of mesenchymal stem cells.
Proc Natl Acad Sci U S A 107(11):4872–4877. 2010. More

Tang J, Peng R, Ding J.
The regulation of stem cell differentiation by cell-cell contact on micropatterned material surfaces.
Biomaterials 31(9):2470–2476. 2010. More

Schauer K, Duong T, Bleakley K, Bardin S, Bornens M, Goud B.
Probabilistic density maps to study global endomembrane organization.
Nat Methods 7(7):560–566. 2010. More

Gao L, McBeath R, Chen CS.
Stem Cell Shape Regulates a Chondrogenic Versus Myogenic Fate Through Rac1 and N-Cadherin.
Stem Cells 2010. More

Raghavan S, Nelson CM, Baranski J, Lim E, Chen CS.
Geometrically controlled endothelial tubulogenesis in micropatterned gels.
Tissue Eng Part A 2010. More

Sillibourne JE, Tack F, Vloemans N, Boeckx A, Thambirajah S, Bonnet P, Ramaekers FC, Bornens M, Grand-Perret T.
Autophosphorylation of polo-like kinase 4 and its role in centriole duplication.
Mol Biol Cell 21(4):547–561. 2010. More

Thery M.
Micropatterning as a tool to decipher cell morphogenesis and functions.
J Cell Sci 123(Pt 24):4201–4213. 2010. More

Tay CY, Yu H, Pal M, Leong WS, Tan NS, Ng KW, Leong DT, Tan LP.
Micropatterned matrix directs differentiation of human mesenchymal stem cells towards myocardial lineage.
Exp Cell Res 316(7):1159–1168. 2010. More



2009
Stybayeva G, Zhu H, Ramanculov E, Dandekar S, George M, Revzin A.
Micropatterned co-cultures of T-lymphocytes and epithelial cells as a model of mucosal immune system.
Biochem Biophys Res Commun 380(3):575–580. 2009. More

Charest JL, Jennings JM, King WP, Kowalczyk AP, Garcia AJ.
Cadherin-mediated cell-cell contact regulates keratinocyte differentiation.
J Invest Dermatol 129(3):564–572. 2009. More

Moon JJ, Hahn MS, Kim I, Nsiah BA, West JL.
Micropatterning of poly(ethylene glycol) diacrylate hydrogels with biomolecules to regulate and guide endothelial morphogenesis.
Tissue Eng Part A 15(3):579–585. 2009. More

Geisse NA, Sheehy SP, Parker KK.
Control of myocyte remodeling in vitro with engineered substrates.
In Vitro Cell Dev Biol Anim 45(7):343–350. 2009. More

Corey JM, Gertz CC, Sutton TJ, Chen Q, Mycek KB, Wang BS, Martin AA, Johnson SL, Feldman EL.
Patterning N-type and S-type neuroblastoma cells with Pluronic F108 and ECM proteins.
J Biomed Mater Res A 2009. More

Doyle AD, Wang FW, Matsumoto K, Yamada KM.
One-dimensional topography underlies three-dimensional fibrillar cell migration.
J Cell Biol 184(4):481–490. 2009. More

Yao L, Wang S, Cui W, Sherlock R, O'Connell C, Damodaran G, Gorman A, Windebank A, Pandit A.
Effect of functionalized micropatterned PLGA on guided neurite growth.
Acta Biomater 5(2):580–588. 2009. More

Curran JM, Chen R, Stokes R, Irvine E, Graham D, Gubbins E, Delaney D, Amro N, Sanedrin R, Jamil H et al.
Nanoscale definition of substrate materials to direct human adult stem cells towards tissue specific populations.
J Mater Sci Mater Med 2009. More

Lee IC, Lee YT, Yu BY, Lai JY, Young TH.
The behavior of mesenchymal stem cells on micropatterned PLLA membranes.
J Biomed Mater Res A 91(3):929–938. 2009. More

Ni M, Tong WH, Choudhury D, Rahim NA, Iliescu C, Yu H.
Cell Culture on MEMS Platforms: A Review.
Int J Mol Sci 10(12):5411–5441. 2009. More



2008
Luo W, Jones SR, Yousaf MN.
Geometric control of stem cell differentiation rate on surfaces.
Langmuir 24(21):12129–12133. 2008. More

Bornens M.
Organelle positioning and cell polarity.
Nat Rev Mol Cell Biol 9(11):874–886. 2008. More

James J, Goluch ED, Hu H, Liu C, Mrksich M.
Subcellular curvature at the perimeter of micropatterned cells influences lamellipodial distribution and cell polarity.
Cell Motil Cytoskeleton 65(11):841–852. 2008. More

S Shah S, Lee JY, Verkhoturov S, Tuleuova N, Schweikert EA, Ramanculov E, Revzin A.
Exercising spatiotemporal control of cell attachment with optically transparent microelectrodes.
Langmuir 24(13):6837–6844. 2008. More

Xia N, Thodeti CK, Hunt TP, Xu Q, Ho M, Whitesides GM, Westervelt R, Ingber DE.
Directional control of cell motility through focal adhesion positioning and spatial control of Rac activation.
FASEB J 22(6):1649–1659. 2008. More

Jinno S, Moeller HC, Chen CL, Rajalingam B, Chung BG, Dokmeci MR, Khademhosseini A.
Microfabricated multilayer parylene-C stencils for the generation of patterned dynamic co-cultures.
J Biomed Mater Res A 86(1):278–288. 2008. More

Schwarzenbacher M, Kaltenbrunner M, Brameshuber M, Hesch C, Paster W, Weghuber J, Heise B, Sonnleitner A, Stockinger H, Schutz GJ.
Micropatterning for quantitative analysis of protein-protein interactions in living cells.
Nat Methods 5(12):1053–1060. 2008. More

Magnusson AK, Linderholm P, Vieider C, Ulfendahl M, Erlandsson A.
Surface protein patterns govern morphology, proliferation, and expression of cellular markers but have no effect on physiological properties of cortical precursor cells.
J Neurosci Res 86(11):2363–2375. 2008. More

Kim SJ, Lee JK, Kim JW, Jung JW, Seo K, Park SB, Roh KH, Lee SR, Hong YH, Kim SJ et al.
Surface modification of polydimethylsiloxane (PDMS) induced proliferation and neural-like cells differentiation of umbilical cord blood-derived mesenchymal stem cells.
J Mater Sci Mater Med 19(8):2953–2962. 2008. More

Pirone DM, Qi L, Colecraft H, Chen CS.
Spatial patterning of gene expression using surface-immobilized recombinant adenovirus.
Biomed Microdevices 10(4):561–566. 2008. More

Thery M, Bornens M.
Get round and stiff for mitosis.
HFSP J 2(2):65–71. 2008. More

Ruiz A, Buzanska L, Ceriotti L, Bretagnol F, Coecke S, Colpo P, Rossi F.
Stem-cell culture on patterned bio-functional surfaces.
J Biomater Sci Polym Ed 19(12):1649–1657. 2008. More



2007
Werbin JL, Heinz WF, Romer LH, Hoh JH.
Micropatterns of an extracellular matrix protein with defined information content.
Langmuir 23(22):10883–10886. 2007. More

Bhadriraju K, Yang M, Alom Ruiz S, Pirone D, Tan J, Chen CS.
Activation of ROCK by RhoA is regulated by cell adhesion, shape, and cytoskeletal tension.
Exp Cell Res 313(16):3616–3623. 2007. More

Yu BY, Chou PH, Chen CA, Sun YM, Kung SS.
C3A cell behaviors on micropatterned chitosan collagen gelatin membranes.
J Biomater Appl 22(3):255–274. 2007. More

Thery M, Jimenez-Dalmaroni A, Racine V, Bornens M, Julicher F.
Experimental and theoretical study of mitotic spindle orientation.
Nature 447(7143):493–496. 2007. More

Hynd MR, Frampton JP, Dowell-Mesfin N, Turner JN, Shain W.
Directed cell growth on protein-functionalized hydrogel surfaces.
J Neurosci Methods 162(1-2):255–263. 2007. More

Chen YM, Shen KC, Gong JP, Osada Y.
Selective cell spreading, proliferation, and orientation on micropatterned gel surfaces.
J Nanosci Nanotechnol 7(3):773–779. 2007. More

Abhyankar VV, Beebe DJ.
Spatiotemporal micropatterning of cells on arbitrary substrates.
Anal Chem 79(11):4066–4073. 2007. More

Zapata P, Su J, Garcia AJ, Meredith JC.
Quantitative high-throughput screening of osteoblast attachment, spreading, and proliferation on demixed polymer blend micropatterns.
Biomacromolecules 8(6):1907–1917. 2007. More

Rundqvist J, Mendoza B, Werbin JL, Heinz WF, Lemmon C, Romer LH, Haviland DB, Hoh JH.
High fidelity functional patterns of an extracellular matrix protein by electron beam-based inactivation.
J Am Chem Soc 129(1):59–67. 2007. More

Rundqvist J, Mendoza B, Werbin JL, Heinz WF, Lemmon C, Romer LH, Haviland DB, Hoh JH.
High fidelity functional patterns of an extracellular matrix protein by electron beam-based inactivation.
J Am Chem Soc 129(1):59–67. 2007. More

Le Beyec J, Xu R, Lee SY, Nelson CM, Rizki A, Alcaraz J, Bissell MJ.
Cell shape regulates global histone acetylation in human mammary epithelial cells.
Exp Cell Res 313(14):3066–3075. 2007. More



2006
Liu WF, Nelson CM, Pirone DM, Chen CS.
E-cadherin engagement stimulates proliferation via Rac1.
J Cell Biol 173(3):431–441. 2006. More

Falconnet D, Csucs G, Grandin HM, Textor M.
Surface engineering approaches to micropattern surfaces for cell-based assays.
Biomaterials 27(16):3044–3063. 2006. More

Thery M, Pepin A, Dressaire E, Chen Y, Bornens M.
Cell distribution of stress fibres in response to the geometry of the adhesive environment.
Cell Motil Cytoskeleton 63(6):341–355. 2006. More

Pirone DM, Liu WF, Ruiz SA, Gao L, Raghavan S, Lemmon CA, Romer LH, Chen CS.
An inhibitory role for FAK in regulating proliferation: a link between limited adhesion and RhoA-ROCK signaling.
J Cell Biol 174(2):277–288. 2006. More

Goffin JM, Pittet P, Csucs G, Lussi JW, Meister JJ, Hinz B.
Focal adhesion size controls tension-dependent recruitment of alpha-smooth muscle actin to stress fibers.
J Cell Biol 172(2):259–268. 2006. More



2005
Thery M, Racine V, Piel M, Pepin A, Dimitrov A, Chen Y, Sibarita JB, Bornens M.
Anisotropy of cell adhesive microenvironment governs cell internal organization and orientation of polarity.
Proc Natl Acad Sci U S A 103(52):19771–19776. 2006. More

Lee JY, Jones C, Zern MA, Revzin A.
Analysis of local tissue-specific gene expression in cellular micropatterns.
Anal Chem 78(24):8305–8312. 2006. More

Thery M, Bornens M.
Cell shape and cell division.
Curr Opin Cell Biol 18(6):648–657. 2006. More

Lussi JW, Falconnet D, Hubbell JA, Textor M, Csucs G.
Pattern stability under cell culture conditions--a comparative study of patterning methods based on PLL-g-PEG background passivation.
Biomaterials 27(12):2534–2541. 2006. More

Thery M, Racine V, Pepin A, Piel M, Chen Y, Sibarita JB, Bornens M.
The extracellular matrix guides the orientation of the cell division axis.
Nat Cell Biol 7(10):947–953. 2005. More

Pasqui D, Rossi A, Barbucci R, Lamponi S, Gerli R, Weber E.
Hyaluronan and sulphated hyaluronan micropatterns: effect of chemical and topographic cues on lymphatic endothelial cell alignment and proliferation.
Lymphology 38(2):50–65. 2005. More

Herndon TM, Pirone DM, Tsokos GC, Chen CS.
T cell-to-T cell clustering enhances NF-kappaB activity by a PI3K signal mediated by Cbl-b and Rho.
Biochem Biophys Res Commun 332(4):1133–1139. 2005. More

Endler EE, Nealey PF, Yin J.
Fidelity of micropatterned cell cultures.
J Biomed Mater Res A 74(1):92–103. 2005. More

Jiang X, Bruzewicz DA, Wong AP, Piel M, Whitesides GM.
Directing cell migration with asymmetric micropatterns.
Proc Natl Acad Sci U S A 102(4):975–978. 2005. More

Pirone DM, Chen CS.
Strategies for engineering the adhesive microenvironment.
J Mammary Gland Biol Neoplasia 9(4):405–417. 2004. More



2004
Leng T, Wu P, Mehenti NZ, Bent SF, Marmor MF, Blumenkranz MS, Fishman HA.
Directed retinal nerve cell growth for use in a retinal prosthesis interface.
Invest Ophthalmol Vis Sci 45(11):4132–4137. 2004. More

Nelson CM, Pirone DM, Tan JL, Chen CS.
Vascular endothelial-cadherin regulates cytoskeletal tension, cell spreading, and focal adhesions by stimulating RhoA.
Mol Biol Cell 15(6):2943–2953. 2004. More

Recknor JB, Recknor JC, Sakaguchi DS, Mallapragada SK.
Oriented astroglial cell growth on micropatterned polystyrene substrates.
Biomaterials 25(14):2753–2767. 2004. More

Thompson DM, Buettner HM.
Oriented Schwann cell monolayers for directed neurite outgrowth.
Ann Biomed Eng 32(8):1120–1130. 2004. More

Vogt AK, Stefani FD, Best A, Nelles G, Yasuda A, Knoll W, Offenhausser A.
Impact of micropatterned surfaces on neuronal polarity.
J Neurosci Methods 134(2):191–198. 2004. More

McBeath R, Pirone DM, Nelson CM, Bhadriraju K, Chen CS.
Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment.
Dev Cell 6(4):483–495. 2004. More

Chen CS.
Using microenvironment to engineer stem cell function.
Conf Proc IEEE Eng Med Biol Soc 7:4964. 2004. More



2003
Oliva AAJ, James CD, Kingman CE, Craighead HG, Banker GA.
Patterning axonal guidance molecules using a novel strategy for microcontact printing.
Neurochem Res 28(11):1639–1648. 2003. More

Brock A, Chang E, Ho CC, LeDuc P, Jiang X, Whitesides GM, Ingber DE.
Geometric determinants of directional cell motility revealed using microcontact printing.
Langmuir 19(5):1611–1617. 2003. More



2002
Hasenbein ME, Andersen TT, Bizios R.
Micropatterned surfaces modified with select peptides promote exclusive interactions with osteoblasts.
Biomaterials 23(19):3937–3942. 2002. More

Wang N, Ostuni E, Whitesides GM, Ingber DE.
Micropatterning tractional forces in living cells.
Cell Motil Cytoskeleton 52(2):97–106. 2002. More



2001
Chen G, Ito Y.
Gradient micropattern immobilization of EGF to investigate the effect of artificial juxtacrine stimulation.
Biomaterials 22(18):2453–2457. 2001. More

Miller C, Shanks H, Witt A, Rutkowski G, Mallapragada S.
Oriented Schwann cell growth on micropatterned biodegradable polymer substrates.
Biomaterials 22(11):1263–1269. 2001. More

Thompson DM, Buettner HM.
Schwann cell response to micropatterned laminin surfaces.
Tissue Eng 7(3):247–265. 2001. More

Miller C, Jeftinija S, Mallapragada S.
Micropatterned Schwann cell-seeded biodegradable polymer substrates significantly enhance neurite alignment and outgrowth.
Tissue Eng 7(6):705–715. 2001. More

Li S, Bhatia S, Hu YL, Shiu YT, Li YS, Usami S, Chien S.
Effects of morphological patterning on endothelial cell migration.
Biorheology 38(2-3):101–108. 2001. More



1990-2000
Dike LE, Chen CS, Mrksich M, Tien J, Whitesides GM, Ingber DE.
Geometric control of switching between growth, apoptosis, and differentiation during angiogenesis using micropatterned substrates.
In Vitro Cell Dev Biol Anim 35(8):441–448. 1999. More

Chen CS, Mrksich M, Huang S, Whitesides GM, Ingber DE.
Micropatterned surfaces for control of cell shape, position, and function.
Biotechnol Prog 14(3):356–363. 1998. More

Chen CS, Mrksich M, Huang S, Whitesides GM, Ingber DE.
Geometric control of cell life and death.
Science 276(5317):1425–1428. 1997. More

Corey JM, Brunette AL, Chen MS, Weyhenmeyer JA, Brewer GJ, Wheeler BC.
Differentiated B104 neuroblastoma cells are a high-resolution assay for micropatterned substrates.
J Neurosci Methods 75(1):91–97. 1997. More

Matsuda T, Chung DJ.
Microfabricated surface designs for cell culture and diagnosis.
ASAIO J 40(3):M594–7. 1994. More

Matsuzawa M, Potember RS, Krauthamer V.
Use of chemically patterned substrate to study directional effect of damaging electrical stimulation on cultured neuroblastoma cells.
Brain Res 667(1):47–53. 1994. More

Clark P, Britland S, Connolly P.
Growth cone guidance and neuron morphology on micropatterned laminin surfaces.
J Cell Sci 105 (Pt 1):203–212. 1993. More

Matsuda T, Sugawara T, Inoue K.
Two-dimensional cell manipulation technology. An artificial neural circuit based on surface microphotoprocessing.
ASAIO J 38(3):M243–7. 1992. More

Britland S, Clark P, Connolly P, Moores G.
Micropatterned substratum adhesiveness: a model for morphogenetic cues controlling cell behavior.
Exp Cell Res 198(1):124–129. 1992. More



1990 and earlier
Rogers SL, McCarthy JB, Palm SL, Furcht LT, Letourneau PC.
Neuron-specific interactions with two neurite-promoting fragments of fibronectin.
J Neurosci 5(2):369–378. 1985. More

Hammarback JA, Palm SL, Furcht LT, Letourneau PC.
Guidance of neurite outgrowth by pathways of substratum-adsorbed laminin.
J Neurosci Res 13(1-2):213–220. 1985. More

Aplin JD, Hughes RC.
Protein-derivatised glass coverslips for the study of cell-to substratum adhesion.
Anal Biochem 113(1):144–148. 1981. More

Letourneau PC.
Cell-to-substratum adhesion and guidance of axonal elongation.
Dev Biol 44(1):92–101. 1975. More

Letourneau PC.
Possible roles for cell-to-substratum adhesion in neuronal morphogenesis.
Dev Biol 44(1):77–91. 1975. More